What are the effects of centrifugation using a centrifuge tube on platelet activity?
Date: 2021-10-27
In vitro, external factors can easily cause platelet activation, and centrifugation in a centrifuge tube can also cause platelet activation and destruction. The purpose of the experiment is not only to obtain PRP with high platelet concentration, but also to keep the obtained platelets in an unactivated state, so that as much growth factor as possible can be released during subsequent use.
P-selectin, also known as CD62P or GMP-140, is a specific marker of platelet activation, and has high specificity compared with the previous PF4, TXB2, etc. When the platelet is activated, on the one hand, the a-granule membrane in the platelet fuses with the open channel system, and CD62P in the a-granule appears on the surface of the platelet membrane, while the platelet surface in the resting state does not express CD62P, so it can pass through the flow The expression rate of CD62P on platelet surface was detected by cytometry to reflect the activation rate of platelets. On the other hand, CD62P can be released into the blood and exist in the plasma in the form of microparticles, becoming the main source of plasma P-selectin, so the detection of plasma P-selectin concentration by E-LASA can also reflect the degree of platelet activation.
Plasma P-selectin concentrations in PRP prepared by 4 different centrifugation methods were detected by ELISA and normalized with corresponding platelet counts. The results of the study found that different centrifugation methods using disposable centrifuge tubes resulted in different concentrations of P-selectin in the prepared PRP platelets. The plasma P-selectin concentration of PRP prepared by centrifugation methods two and four was lower than that of centrifugation methods one and three, and the concentration of centrifugation method two was slightly lower than that of centrifugation method four. The results showed that PRP prepared by centrifugation method 2 not only had higher platelet recovery rate and platelet concentration, but also had little effect on platelet activity.
P-selectin, also known as CD62P or GMP-140, is a specific marker of platelet activation, and has high specificity compared with the previous PF4, TXB2, etc. When the platelet is activated, on the one hand, the a-granule membrane in the platelet fuses with the open channel system, and CD62P in the a-granule appears on the surface of the platelet membrane, while the platelet surface in the resting state does not express CD62P, so it can pass through the flow The expression rate of CD62P on platelet surface was detected by cytometry to reflect the activation rate of platelets. On the other hand, CD62P can be released into the blood and exist in the plasma in the form of microparticles, becoming the main source of plasma P-selectin, so the detection of plasma P-selectin concentration by E-LASA can also reflect the degree of platelet activation.
Plasma P-selectin concentrations in PRP prepared by 4 different centrifugation methods were detected by ELISA and normalized with corresponding platelet counts. The results of the study found that different centrifugation methods using disposable centrifuge tubes resulted in different concentrations of P-selectin in the prepared PRP platelets. The plasma P-selectin concentration of PRP prepared by centrifugation methods two and four was lower than that of centrifugation methods one and three, and the concentration of centrifugation method two was slightly lower than that of centrifugation method four. The results showed that PRP prepared by centrifugation method 2 not only had higher platelet recovery rate and platelet concentration, but also had little effect on platelet activity.
