What is the difference between virus preservation solution and cell preservation solution
Date: 2021-10-27
What is virus preservation solution? What is cell preservation solution? I believe many people can't tell the difference between the two? What is the purpose of these two? Let's find out together!
Components and uses of virus preservation solution
The virus preservation solution is a kind of liquid that protects the virus to be detected by dipping the swab virus sample in the sampling tube. It can collect throat swabs, nasal swabs or tissue samples from specific parts, and the stored samples can be used for subsequent nucleic acid extraction or purification. and other clinical trials. It is suitable for collection, preservation and transportation of various common virus samples. The virus preservation solution is also divided into two types: inactivated and non-inactivated!
Non-inactivated version: does not contain lysate, can maintain the activity and integrity of pathogens, and can be used for virus culture and isolation.
Inactivated version: It can instantly lyse pathogens to release nucleic acid, and the protective agent can prevent nucleic acid from being degraded.
Components and uses of cell preservation solution
The so-called cell preservation solution is a general-purpose cell cryopreservation solution. Cell cryopreservation is an important technical means for cell culture, seeding, seed preservation and ensuring the smooth progress of experiments. The basic principle of cell cryopreservation and recovery is slow freezing. Fast thawing, experiments have shown that this can preserve cell viability. At present, glycerol or dimethyl sulfoxide is used as a protective agent for cell cryopreservation. These two substances can improve the permeability of the cell membrane to water. In addition, slow freezing can make the water in the cell leak out of the cell and reduce the ice crystal in the cell. formation, thereby reducing cell damage due to ice crystal formation in cryopreservation of cells. The composition of commonly used cryopreservation solution is as follows: 20% serum (FBS), 10% DMSO, 70% 1640 medium; or 90% serum (FBS), 10% DMSO, which can prevent the formation of intracellular ice crystals. DMSO and FBS were added to DMEM, the content of each accounting for 10% of the total volume, and then filtered through a filter membrane and stored in aliquots for later use.
Components and uses of virus preservation solution
The virus preservation solution is a kind of liquid that protects the virus to be detected by dipping the swab virus sample in the sampling tube. It can collect throat swabs, nasal swabs or tissue samples from specific parts, and the stored samples can be used for subsequent nucleic acid extraction or purification. and other clinical trials. It is suitable for collection, preservation and transportation of various common virus samples. The virus preservation solution is also divided into two types: inactivated and non-inactivated!
Non-inactivated version: does not contain lysate, can maintain the activity and integrity of pathogens, and can be used for virus culture and isolation.
Inactivated version: It can instantly lyse pathogens to release nucleic acid, and the protective agent can prevent nucleic acid from being degraded.
Components and uses of cell preservation solution
The so-called cell preservation solution is a general-purpose cell cryopreservation solution. Cell cryopreservation is an important technical means for cell culture, seeding, seed preservation and ensuring the smooth progress of experiments. The basic principle of cell cryopreservation and recovery is slow freezing. Fast thawing, experiments have shown that this can preserve cell viability. At present, glycerol or dimethyl sulfoxide is used as a protective agent for cell cryopreservation. These two substances can improve the permeability of the cell membrane to water. In addition, slow freezing can make the water in the cell leak out of the cell and reduce the ice crystal in the cell. formation, thereby reducing cell damage due to ice crystal formation in cryopreservation of cells. The composition of commonly used cryopreservation solution is as follows: 20% serum (FBS), 10% DMSO, 70% 1640 medium; or 90% serum (FBS), 10% DMSO, which can prevent the formation of intracellular ice crystals. DMSO and FBS were added to DMEM, the content of each accounting for 10% of the total volume, and then filtered through a filter membrane and stored in aliquots for later use.
